determination of 5-hydroxymethyl-2-furaldehyde of crude and processed fructus corni in freely moving rats using in vivo microdialysis sampling and liquid chromatography

the purpose of this study was to develop a sensitive and fast microdialysis coupled with high-performance liquid chromatographic (hplc) method for determination of 5-hydroxymethyl-2-furaldehyde (5-hmf) in free-moving rats after i.g. administration of the aqueous extract of crud fructus corni and its processed products of jiuzheng pin (jzp). the concentration of 5-hmf in free-moving rats after i.g. administration of the water extract of fructus corni and jzp was analyzed by microdialysis coupled with hplc. results demonstrated that the concentration of 5-hmf in microdialysate was 1.4951μg/l, but higher in rat microdialysate after i.g. administration of the aqueous extract of jzp (5.2662 μg/l). in conclusion, this method is proved to be rapid，accurate and simple. real-time in vivo monitoring the concentration of 5-hmf provides the theoretical basis for further explaining the processing mechanism of f. corni.